Transient transfection and expression of firefly luciferase in Giardia lamblia.

Transient transfection and expression of firefly luciferase in Giardia lamblia.

We have developed a gene transfer system for the protozoan parasite Giardia lamblia. This organism is responsible for many cases of diarrhea worldwide and is considered to be one of the most primitive eukaryotes. Expression of a heterologous gene was detected in this parasite after electroporation with appropriate DNA constructs. We constructed a series of transfection plasmids using flanking s...

Giardia Lamblia.

AXENIC CULTURE AND CRYOPRESERVATION OF GIARDIA LAMBLIA ISOLATED IN IRAN

Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...

Giardia lamblia and Giardiasis

Introduction Ask the average outdoors person about Giardia lamblia, and they have certainly heard about it. Almost always, however, they are considerably misinformed about both the organism’s significance in wilderness water and the seriousness of the disease, giardiasis, if contracted. The amount of information easily found on the subject is voluminous. Unfortunately, almost all of it is flawe...

Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

A cDNA library was constructed from firefly (Photinus pyralis) lantern poly(A)+ RNA, using the Escherichia coli expression vector lambda gt11. The library was screened with anti-P. pyralis luciferase (Photinus luciferin:oxygen 4-oxidoreductase, EC 1.13.12.7) antibody, and several cDNA clones expressing luciferase antigens were isolated. One clone, lambda Luc1, contained 1.5 kilobase pairs of cD...